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Article Dans Une Revue Journal of Nanoparticle Research Année : 2017

Large-scale protein/antibody patterning with limiting unspecific adsorption

Résumé

A simple synthetic route based on nanosphere lithography has been developed in order to design largescale nanoarray for specific control of protein anchoring. This technique based on two-dimensional (2D) colloidal crystals composed of polystyrene spheres allows the easy and inexpensive fabrication of large arrays (up to several centimeters) by reducing the cost. A silicon wafer coated with a thin adhesion layer of chromium (15 nm) and a layer of Gold (50 nm) is used as a substrate. PS spheres are deposited on the gold surface using the floating-transferring technique. The PS spheres were then functionalized with PEG-biotin and the defects by self-assembly monolayer (SAMs) PEG to prevent unspecific adsorption. Using epifluorescence microscopy, we show that after immersion of sample on target protein (avidin and anti-avidin) solution, the latter are specifically located on polystyrene spheres. Thus, these results are meaningful for exploration of devices based on large-scale nanoarray of PS spheres and can be used for detection of target proteins or simply to pattern a surface with specific proteins.

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Chimie
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Dates et versions

hal-01674778 , version 1 (10-06-2021)

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Viktoriia Fedorenko, Mikhael Bechelany, Jean-Marc Janot, Valentyn Smyntyna, Sébastien Balme. Large-scale protein/antibody patterning with limiting unspecific adsorption. Journal of Nanoparticle Research, 2017, 19 (10), ⟨10.1007/s11051-017-4053-x⟩. ⟨hal-01674778⟩
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