The Syp1/FCHo2 protein induces septin filament bundling through its intrinsically disordered domain - Université de Montpellier Accéder directement au contenu
Article Dans Une Revue Cell Reports Année : 2022

The Syp1/FCHo2 protein induces septin filament bundling through its intrinsically disordered domain

Résumé

The septin collar of budding yeast is an ordered array of septin filaments that serves a scaffolding function for the cytokinetic machinery at the bud neck and compartmentalizes the membrane between mother and daughter cell. How septin architecture is aided by septin-binding proteins is largely unknown. Syp1 is an endocytic protein that was implicated in the timely recruitment of septins to the newly forming collar through an unknown mechanism. Using advanced microscopy and in vitro reconstitution assays, we show that Syp1 is able to align laterally and tightly pack septin filaments, thereby forming flat bundles or sheets. This property is shared by the Syp1 mammalian counterpart FCHo2, thus emphasizing conserved protein functions. Interestingly, the septin-bundling activity of Syp1 resides mainly in its intrinsically disordered region. Our data uncover the mechanism through which Syp1 promotes septin collar assembly and offer another example of functional diversity of unstructured protein domains.
Fichier principal
Vignette du fichier
2022-Cell report.pdf (7.9 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Licence : CC BY NC ND - Paternité - Pas d'utilisation commerciale - Pas de modification

Dates et versions

hal-04114167 , version 1 (01-06-2023)

Licence

Paternité - Pas d'utilisation commerciale - Pas de modification

Identifiants

Citer

Sandy Ibanes, Fatima El Alaoui, Josephine Lai Kee Him, Chantal Cazevieille, François Hoh, et al.. The Syp1/FCHo2 protein induces septin filament bundling through its intrinsically disordered domain. Cell Reports, 2022, 41 (10), pp.111765. ⟨10.1016/j.celrep.2022.111765⟩. ⟨hal-04114167⟩
14 Consultations
14 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More