, For the retinal layer thickness measurement, use the automated segmentation software provided by the manufacturer

, Click on the "Patient

, Load the desired OCT data onto the OCT software by right-clicking on the folder icon in the desired scan

, Right click on the B-scan; configure the calipers by enabling up to 10 measuring calipers

, Choosing the desired caliper by a right-clicking on the B-scan; place it accordingly onto the retina for measurement. NOTE: For the scans centered on the ONH, set 5 calipers on each side of it, equidistant from each other. For the peripapillary analysis, make sure that the caliper is not placed too far from the ONH. For radial scanning, analyze 10 pictures per scan that were chosen by the OCT software

, Save the results for analysis in a spreadsheet software program by right-clicking on the B-scan and clicking "Save results". NOTE: The results can be found in the same folder as the scanning data

, MRI Retina Tool", developed for an open-source image processing program (see the Table of Materials)

, Make sure that the "MRI Retina Tool" and the "Modify Polygon Section Tool" macros are active. Load the image. Click on the m button to start the measurement

W. Drexler and J. G. Fujimoto, State-of-the-art retinal optical coherence tomography, Prog Retin Eye Res, vol.27, issue.1, pp.45-88, 2008.

J. Grenier, WFS1 in Optic Neuropathies: Mutation Findings in Nonsyndromic Optic Atrophy and Assessment of Clinical Severity, Ophthalmology, vol.123, issue.9, pp.1989-1998, 2016.
URL : https://hal.archives-ouvertes.fr/hal-01864083

A. Zmyslowska, Retinal thinning as a marker of disease progression in patients with Wolfram syndrome, Diabetes Care, vol.38, issue.3, pp.36-37, 2015.

M. D. Fischer, Noninvasive, in vivo assessment of mouse retinal structure using optical coherence tomography, PLoS One, vol.4, issue.10, p.7507, 2009.

K. Grieve, O. Thouvenin, A. Sengupta, V. M. Borderie, and M. Paques, Appearance of the Retina With Full-Field Optical Coherence Tomography, Invest Ophthalmol Vis Sci, vol.57, issue.9, pp.96-104, 2016.
URL : https://hal.archives-ouvertes.fr/hal-01390871

B. Chang, Retinal degeneration mutants in the mouse, Vision Res, vol.42, issue.4, pp.517-525, 2002.

S. Mustafa and L. Pandit, Approach to diagnosis and management of optic neuropathy, Neurol India, vol.62, issue.6, pp.599-605, 2014.

E. Sarzi, The human OPA1delTTAG mutation induces premature age-related systemic neurodegeneration in mouse, Brain, vol.135, pp.3599-3613, 2012.

E. Sarzi, Increased steroidogenesis promotes early-onset and severe vision loss in females with OPA1 dominant optic atrophy, Hum Mol Genet, vol.25, issue.12, pp.2539-2551, 2016.
URL : https://hal.archives-ouvertes.fr/hal-01867305

C. Delettre-cribaillet, C. P. Hamel, and G. Lenaers, | Lenaers, G. et al. Dominant optic atrophy, Orphanet J Rare Dis, vol.7, issue.46, 2007.

G. Lenaers, Dominant optic atrophy, Orphanet J Rare Dis, vol.7, issue.46, 2012.
URL : https://hal.archives-ouvertes.fr/inserm-00767364

C. Delettre, Nuclear gene OPA1, encoding a mitochondrial dynamin-related protein, is mutated in dominant optic atrophy, Nat Genet, vol.26, issue.2, pp.207-210, 2000.

Y. Liu, Monitoring retinal morphologic and functional changes in mice following optic nerve crush, Invest Ophthalmol Vis Sci, vol.55, issue.6, pp.3766-3774, 2014.