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Synergistic action of vasopressin and aldosterone on basolateral Na+-K+-ATPase in the cortical collecting duct

Abstract : The respective effects of aldosterone and ar-ginine vasopressin (AVP) were examined on the number of active Na+-K+-ATPase and their pumping activity in nonperfused microdissected mouse cortical collecting tu-bules (CCD) by measuring specific 3H-ouabain binding and ouabain-sensitive 86Rb uptake. In adrenalectomized (ADX) animals, incubation of CCD with AVP (10-8 for 5 min) had no effect on the number of pumps. In contrast, in ADX animals replete with aldosterone, AVP induced a-=40% increase in the number of pumps. This was accompanied by a-_-60-65% increase in ouabain-sensitive Rb uptake. AVP effect was dose-dependent (10-1~-8 M) and was reproduced by dDAVP, forsko-lin and 8-Br cAMP, indicating a V 2 pathway. It was inhibited by amiloride 10-5 M, and did not occur in CCD incubated in hyperosmotic solution, suggesting that the signal was transmitted via apical sodium entry and cell swelling. Finally, the AVP-dependent increase in the number of pumps was rapid (within 5 min) and transient (<25 rain). These results demonstrate that, in the CCD, aldoste-rone and AVP act synergistically to increase not only the apical sodium entry but also the basolateral Na+-K +-ATPase transport capacity: AVP allows a rapid recruitment and/or activation of an aldosterone-dependent pool of latent Na+-K+-ATPase.
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Soumis le : mardi 18 février 2020 - 18:12:02
Dernière modification le : jeudi 20 février 2020 - 01:37:13

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N. Coutry, N. Farman, J.P. Bonvalet, M. Blot-Chabaud. Synergistic action of vasopressin and aldosterone on basolateral Na+-K+-ATPase in the cortical collecting duct. Journal of Membrane Biology, Springer Verlag (Germany), 1995, 145 (1), pp.99 - 106. ⟨10.1007/bf00233310⟩. ⟨hal-02477984⟩

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