Differential Susceptibility and Innate Immune Response of Aedes aegypti and Aedes albopictus to the Haitian Strain of the Mayaro Virus - Université de Montpellier Accéder directement au contenu
Article Dans Une Revue Viruses Année : 2019

Differential Susceptibility and Innate Immune Response of Aedes aegypti and Aedes albopictus to the Haitian Strain of the Mayaro Virus

Cecile Baronti
  • Fonction : Auteur
  • PersonId : 971542
Rodolphe Hamel
Loïc Talignani
Florian Liegeois
Ronald E Morales Vargas
  • Fonction : Auteur
  • PersonId : 1060062
Antoine Nougairède
  • Fonction : Auteur
  • PersonId : 1034833

Résumé

Mayaro (MAYV) is an emerging arthropod-borne virus belonging to the Alphavirus genus of the Togaviridae family. Although forest-dwelling Haemagogus mosquitoes have been considered as its main vector, the virus has also been detected in circulating Aedes ssp mosquitoes. Here we assess the susceptibility of Aedes aegypti and Aedes albopictus to infection with MAYV and their innate immune response at an early stage of infection. Aedes albopictus was more susceptible to infection with MAYV than Ae. aegypti. Analysis of transcript levels of twenty immunity-related genes by real-time PCR in the midgut of both mosquitoes infected with MAYV revealed increased expression of several immune genes, including CLIP-domain serine proteases, the anti-microbial peptides defensin A, E, cecropin E, and the virus inducible gene. The regulation of certain genes appeared to be Aedes species-dependent. Infection of Ae. aegypti with MAYV resulted in increased levels of myeloid differentiation2-related lipid recognition protein (ML26A) transcripts, as compared to Ae. albopictus. Increased expression levels of thio-ester-containing protein 22 (TEP22) and Niemann-Pick type C1 (NPC1) gene transcripts were observed in infected Ae. albopictus, but not Ae. aegypti. The differences in these gene expression levels during MAYV infection could explain the variation in susceptibility observed in both mosquito species.
Fichier principal
Vignette du fichier
Misse_01.pdf (994.63 Ko) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-02396716 , version 1 (06-12-2019)

Licence

Paternité

Identifiants

Citer

Fode Diop, Haoues Alout, Cheikh Tidiane Diagne, Michèle Bengue, Cecile Baronti, et al.. Differential Susceptibility and Innate Immune Response of Aedes aegypti and Aedes albopictus to the Haitian Strain of the Mayaro Virus. Viruses, 2019, 11 (10), pp.924. ⟨10.3390/v11100924⟩. ⟨hal-02396716⟩
196 Consultations
138 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More