Next-Generation Sequencing on Insectivorous Bat Guano: An Accurate Tool to Identify Arthropod Viruses of Potential Agricultural Concern

Abstract : Viruses belonging to the Dicistroviridae family have attracted a great deal of attention from scientists owing to their negative impact on agricultural economics, as well as their recent identification as potential aetiological agents of febrile illness in human patients. On the other hand, some Dicistroviruses are also studied for their potential biopesticide properties. To date, Dicistrovirus characterized in African mainland remain scarce. By using High-Throughput Sequencing technology on insectivorous bat faeces (Hipposideros Caffer) sampled in a cave used by humans to collect bat guano (bat manure) as fertilizer in Zimbabwe, we characterized the full-length sequences of three Dicistrovirus belonging to the Cripavirus and Aparavirus genus: Big Sioux River Virus-Like (BSRV-Like), Acute Bee Paralysis Virus (ABPV), and Aphid Lethal Paralysis Virus (ALPV). Phylogenetic analyses of ORF-1 and ORF-2 genes showed a complex evolutionary history between BSRV and close viruses, as well as for the Aparavirus genus. Herewith, we provide the first evidence of the presence of Dicistrovirus in Zimbabwe and highlight the need to further document the impact of such viruses on crops, as well as in beekeeping activities in Zimbabwe which represent a crucial source of income for Zimbabwean people.
Liste complète des métadonnées

Littérature citée [59 références]  Voir  Masquer  Télécharger

https://hal.umontpellier.fr/hal-02398831
Contributeur : Florian Liegeois <>
Soumis le : dimanche 8 décembre 2019 - 09:39:06
Dernière modification le : jeudi 20 février 2020 - 19:23:47

Fichier

viruses-11-01102.pdf
Fichiers produits par l'(les) auteur(s)

Identifiants

Collections

Citation

Mathieu Bourgarel, Valérie Noël, Davies Pfukenyi, Johan Michaux, Adrien André, et al.. Next-Generation Sequencing on Insectivorous Bat Guano: An Accurate Tool to Identify Arthropod Viruses of Potential Agricultural Concern. Viruses, MDPI, 2019, 11 (12), pp.1102. ⟨10.3390/v11121102⟩. ⟨hal-02398831⟩

Partager

Métriques

Consultations de la notice

39

Téléchargements de fichiers

78