Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

Chaperone-mediated ordered assembly of the SAGA and NuA4 transcription co-activator complexes in yeast

Abstract : Transcription initiation involves the coordinated activities of large multimeric complexes, but little is known about their biogenesis. Here we report several principles underlying the assembly and topological organization of the highly conserved SAGA and NuA4 co-activator complexes, which share the Tra1 subunit. We show that Tra1 contributes to the overall integrity of NuA4, whereas, within SAGA, it specifically controls the incorporation of the de-ubiquitination module (DUB), as part of an ordered assembly pathway. Biochemical and functional analyses reveal the mechanism by which Tra1 specifically interacts with either SAGA or NuA4. Finally, we demonstrate that Hsp90 and its cochaperone TTT promote Tra1 de novo incorporation into both complexes, indicating that Tra1, the sole pseudokinase of the PIKK family, shares a dedicated chaperone machinery with its cognate kinases. Overall, our work brings mechanistic insights into the assembly of transcriptional complexes and reveals the contribution of dedicated chaperones to this process.
Liste complète des métadonnées

Littérature citée [70 références]  Voir  Masquer  Télécharger

https://hal.umontpellier.fr/hal-02394906
Contributeur : Yvan Boublik <>
Soumis le : vendredi 13 mars 2020 - 10:33:02
Dernière modification le : mardi 17 mars 2020 - 17:53:53

Fichier

MS-2019-Elías-Villalobos.pdf
Fichiers éditeurs autorisés sur une archive ouverte

Licence


Distributed under a Creative Commons Paternité 4.0 International License

Identifiants

Collections

Citation

Alberto Elías-Villalobos, Damien Toullec, Céline Faux, Martial Séveno, Dominique Helmlinger. Chaperone-mediated ordered assembly of the SAGA and NuA4 transcription co-activator complexes in yeast. Nature Communications, Nature Publishing Group, 2019, 10 (1), ⟨10.1038/s41467-019-13243-w⟩. ⟨hal-02394906⟩

Partager

Métriques

Consultations de la notice

59

Téléchargements de fichiers

50