L. Rajagopal, A. Clancy, and C. E. Rubens, A eukaryotic type serine/threonine kinase and phosphatase in Streptococcus agalactiae reversibly phosphorylate an inorganic pyrophosphatase and affect growth, cell segregation, and virulence, J Biol Chem, vol.278, pp.14429-14441, 2003.

H. Hussain, P. Branny, A. , and E. , A eukaryotic-type serine/threonine protein kinase is required for biofilm formation, genetic competence, and acid resistance in Streptococcus mutans, J Bacteriol, vol.188, pp.1628-1632, 2006.

J. Echenique, A. Kadioglu, S. Romao, P. W. Andrew, and M. C. Trombe, Protein serine/threonine kinase StkP positively controls virulence and competence in Streptococcus pneumoniae, Infect Immun, vol.72, pp.2434-2437, 2004.

H. Jin and V. Pancholi, Identification and biochemical characterization of a eukaryotic-type serine/threonine kinase and its cognate phosphatase in Streptococcus pyogenes: their biological functions and substrate identification, J Mol Biol, vol.357, pp.1351-1372, 2006.

Y. Av-gay, E. , and M. , The eukaryotic-like Ser/Thr protein kinases of Mycobacterium tuberculosis, Trends Microbiol, vol.8, pp.238-244, 2000.

R. Chaba, M. Raje, and P. K. Chakraborti, Evidence that a eukaryotic-type serine/threonine protein kinase from Mycobacterium tuberculosis regulates morphological changes associated with cell division, Eur J Biochem, vol.269, pp.1078-1085, 2002.

A. Wehenkel, M. Bellinzoni, M. Grana, R. Duran, A. Villarino et al., Mycobacterial Ser/Thr protein kinases and phosphatases: physiological roles and therapeutic potential, Biochim Biophys Acta, vol.1784, pp.193-202, 2008.
URL : https://hal.archives-ouvertes.fr/pasteur-00508942

V. Molle and L. Kremer, Division and cell envelope regulation by Ser/Thr phosphorylation: Mycobacterium shows the way, Mol Microbiol, vol.75, pp.1064-1077, 2010.
URL : https://hal.archives-ouvertes.fr/hal-02282762

T. Alber, Signaling mechanisms of the Mycobacterium tuberculosis receptor Ser/Thr protein kinases, Curr Opin Struct Biol, vol.19, pp.650-657, 2009.

A. E. Greenstein, C. Grundner, N. Echols, L. M. Gay, T. N. Lombana et al., Structure/function studies of Ser/Thr and Tyr protein phosphorylation in Mycobacterium tuberculosis, J Mol Microbiol Biotechnol, vol.9, pp.167-181, 2005.

E. E. Galyov, S. Hakansson, A. Forsberg, and H. Wolf-watz, A secreted protein kinase of Yersinia pseudotuberculosis is an indispensable virulence determinant, Nature, vol.361, pp.730-732, 1993.

S. Hakansson, E. E. Galyov, R. Rosqvist, and H. Wolf-watz, The Yersinia YpkA Ser/Thr kinase is translocated and subsequently targeted to the inner surface of the HeLa cell plasma membrane, Mol Microbiol, vol.20, pp.593-603, 1996.

C. Archambaud, E. Gouin, J. Pizarro-cerda, P. Cossart, and O. Dussurget, Translation elongation factor EF-Tu is a target for Stp, a serine-threonine phosphatase involved in virulence of Listeria monocytogenes, Mol Microbiol, vol.56, pp.383-396, 2005.

A. Lima, R. Duran, G. E. Schujman, M. J. Marchissio, M. M. Portela et al., Serine/threonine protein kinase PrkA of the human pathogen Listeria monocytogenes: biochemical characterization and identification of interacting partners through proteomic approaches, J Proteomics, vol.74, pp.1720-1734, 2011.
URL : https://hal.archives-ouvertes.fr/pasteur-00685058

J. Wang, C. Li, H. Yang, A. Mushegian, J. et al., A novel serine/threonine protein kinase homologue of Pseudomonas aeruginosa is specifically inducible within the host infection site and is required for full virulence in neutropenic mice, J Bacteriol, vol.180, pp.6764-6768, 1998.

C. J. Kristich, C. L. Wells, and G. M. Dunny, A eukaryotic-type Ser/Thr kinase in Enterococcus faecalis mediates antimicrobial resistance and intestinal persistence, Proc Natl Acad Sci U S A, vol.104, pp.3508-3513, 2007.

, No reuse allowed without permission. (which was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. The copyright holder for this preprint, 2019.

A. M. Beltramini, C. D. Mukhopadhyay, and V. Pancholi, Modulation of cell wall structure and antimicrobial susceptibility by a Staphylococcus aureus eukaryote-like serine/threonine kinase and phosphatase, Infect Immun, vol.77, pp.1406-1416, 2009.

Q. C. Truong-bolduc, Y. Ding, and D. C. Hooper, Posttranslational modification influences the effects of MgrA on norA expression in Staphylococcus aureus, J Bacteriol, vol.190, pp.7375-7381, 2008.

Q. C. Truong-bolduc and D. C. Hooper, Phosphorylation of MgrA and its effect on expression of the NorA and NorB efflux pumps of Staphylococcus aureus, J Bacteriol, vol.192, pp.2525-2534, 2010.

L. Gannoun-zaki, L. Patzold, S. Huc-brandt, G. Baronian, M. I. Elhawy et al., PtpA, a secreted tyrosine phosphatase from Staphylococcus aureus, contributes to virulence and interacts with coronin-1A during infection, J Biol Chem, vol.293, pp.15569-15580, 2018.
URL : https://hal.archives-ouvertes.fr/hal-02281747

M. J. Canova and V. Molle, Bacterial serine/threonine protein kinases in host-pathogen interactions, J Biol Chem, vol.289, pp.9473-9479, 2014.
URL : https://hal.archives-ouvertes.fr/hal-02282652

J. Kazar, Coxiella burnetii infection, Ann N Y Acad Sci, vol.1063, pp.105-114, 2005.

N. Arricau-bouvery and A. Rodolakis, Is Q fever an emerging or re-emerging zoonosis?, Vet Res, vol.36, pp.327-349, 2005.
URL : https://hal.archives-ouvertes.fr/hal-00902979

M. G. Madariaga, K. Rezai, G. M. Trenholme, and R. A. Weinstein, Q fever: a biological weapon in your backyard, Lancet Infect Dis, vol.3, pp.709-721, 2003.

A. Luhrmann, C. V. Nogueira, K. L. Carey, C. R. Roy, L. Klingenbeck et al., The Coxiella burnetii type IV secretion system substrate CaeB inhibits intrinsic apoptosis at the mitochondrial level, Proc Natl Acad Sci U S A, vol.107, pp.675-687, 2010.

L. D. Cunha, J. M. Ribeiro, T. D. Fernandes, L. M. Massis, C. A. Khoo et al., Inhibition of inflammasome activation by Coxiella burnetii type IV secretion system effector IcaA, Nat Commun, vol.6, p.10205, 2015.

C. L. Larson, P. A. Beare, D. E. Voth, D. Howe, D. C. Cockrell et al., Coxiella burnetii effector proteins that localize to the parasitophorous vacuole membrane promote intracellular replication, Infect Immun, vol.83, pp.661-670, 2015.

C. L. Larson, P. A. Beare, D. Howe, and R. A. Heinzen, Coxiella burnetii effector protein subverts clathrin-mediated vesicular trafficking for pathogen vacuole biogenesis, Proc Natl Acad Sci U S A, vol.110, pp.4770-4779, 2013.

J. P. Vogel, Turning a tiger into a house cat: using Legionella pneumophila to study Coxiella burnetii, Trends Microbiol, vol.12, pp.103-105, 2004.

K. L. Carey, H. J. Newton, A. Luhrmann, and C. R. Roy, The Coxiella burnetii Dot/Icm system delivers a unique repertoire of type IV effectors into host cells and is required for intracellular replication, PLoS Pathog, vol.7, p.1002056, 2011.

C. Chen, S. Banga, K. Mertens, M. M. Weber, I. Gorbaslieva et al., Large-scale identification and translocation of type IV secretion substrates by Coxiella burnetii, Proc Natl Acad Sci U S A, vol.107, pp.21755-21760, 2010.

H. J. Newton, J. A. Mcdonough, and C. R. Roy, Effector protein translocation by the Coxiella burnetii Dot/Icm type IV secretion system requires endocytic maturation of the pathogen-occupied vacuole, PLoS One, vol.8, p.54566, 2013.

S. K. Hanks, A. M. Quinn, and T. Hunter, The protein kinase family: conserved features and deduced phylogeny of the catalytic domains, Science, vol.241, pp.42-52, 1988.

S. K. Hanks and T. Hunter, Protein kinases 6. The eukaryotic protein kinase superfamily: kinase (catalytic) domain structure and classification, Faseb J, vol.9, pp.576-596, 1995.

C. Noroy, T. Lefrancois, D. F. Meyer, Z. Lifshitz, D. Burstein et al., Searching Algorithm for Type IV Effector proteins (S4TE) 2.0: improved tools for type IV effector prediction, analysis and comparison. bioRxiv 37, Proc Natl Acad Sci U S A, vol.110, pp.707-715, 2013.

E. Martinez, J. Allombert, F. Cantet, A. Lakhani, N. Yandrapalli et al., Coxiella burnetii effector CvpB modulates phosphoinositide metabolism for optimal vacuole development, Proc Natl Acad Sci U S A, vol.113, pp.3260-3269, 2016.
URL : https://hal.archives-ouvertes.fr/hal-01999389

M. Fiuza, M. J. Canova, I. Zanella-cleon, M. Becchi, A. J. Cozzone et al., From the characterization of the four serine/threonine protein kinases 11 (PknA/B/G/L) of Corynebacterium glutamicum toward the role of PknA and PknB in cell division, J Biol Chem, vol.283, pp.18099-18112, 2008.

V. Molle, J. Leiba, I. Zanella-cleon, M. Becchi, and L. Kremer, An improved method to unravel phosphoacceptors in Ser/Thr protein kinase-phosphorylated substrates, Proteomics, vol.10, pp.3910-3915, 2010.
URL : https://hal.archives-ouvertes.fr/hal-02282736

E. Martinez, F. Cantet, M. ;. Bonazzi, M. E. Harbour, D. Tattersall et al., Membrane recruitment of the cargo-selective retromer subcomplex is catalysed by the small GTPase Rab7 and inhibited by the Rab-GAP TBC1D5, Journal of visualized experiments : JoVE, e52851 42. Seaman, vol.122, pp.2371-2382, 2009.

M. Borg-distefano, L. Hofstad-haugen, Y. Wang, H. Perdreau-dahl, I. Kjos et al., TBC1D5 controls the GTPase cycle of Rab7b, Journal of cell science, p.131, 2018.

D. Popovic and I. Dikic, TBC1D5 and the AP2 complex regulate ATG9 trafficking and initiation of autophagy, EMBO reports, vol.15, pp.392-401, 2014.

A. Omsland, P. A. Beare, J. Hill, D. C. Cockrell, D. Howe et al., Isolation from animal tissue and genetic transformation of Coxiella burnetii are facilitated by an improved axenic growth medium, Appl Environ Microbiol, vol.77, pp.3720-3725, 2011.

D. J. Manstein, H. P. Schuster, P. Morandini, and D. M. Hunt, Cloning vectors for the production of proteins in Dictyostelium discoideum, Gene, vol.162, pp.129-134, 1995.

L. Alibaud, P. Cosson, and M. Benghezal, Dictyostelium discoideum transformation by oscillating electric field electroporation, BioTechniques, vol.35, pp.82-73, 2003.

K. Barlocher, C. A. Hutter, A. L. Swart, B. Steiner, A. Welin et al., Structural insights into Legionella RidL-Vps29 retromer subunit interaction reveal displacement of the regulator TBC1D5, Nat Commun, vol.8, p.1543, 2017.
URL : https://hal.archives-ouvertes.fr/hal-02281984

E. Martinez, F. Cantet, L. Fava, I. Norville, and M. Bonazzi, Identification of OmpA, a Coxiella burnetii protein involved in host cell invasion, by multi-phenotypic high-content screening, PLoS Pathog, vol.10, p.1004013, 2014.
URL : https://hal.archives-ouvertes.fr/hal-01999429

P. A. Beare, K. M. Sandoz, A. Omsland, D. D. Rockey, and R. A. Heinzen, Advances in genetic manipulation of obligate intracellular bacterial pathogens, Frontiers in microbiology, vol.2, p.97, 2011.

V. Molle, L. Kremer, C. Girard-blanc, G. S. Besra, A. J. Cozzone et al., An FHA phosphoprotein recognition domain mediates protein EmbR phosphorylation by PknH, a Ser/Thr protein kinase from Mycobacterium tuberculosis, Biochemistry, vol.42, pp.15300-15309, 2003.
URL : https://hal.archives-ouvertes.fr/hal-00313196

E. Charpentier, A. I. Anton, P. Barry, B. Alfonso, Y. Fang et al., Novel cassettebased shuttle vector system for gram-positive bacteria, Appl Environ Microbiol, vol.70, pp.6076-6085, 2004.