Basolateral internalization of GPIanchored proteins occurs via a clathrin-independent flotillin-dependent pathway in polarized hepatic cells, Molecular biology of the cell, vol.20, pp.3792-3800, 2009. ,
URL : https://hal.archives-ouvertes.fr/hal-00579694
, , 2012.
, Flotillin-1/reggie-2 protein plays dual role in activation of receptor-tyrosine kinase/mitogenactivated protein kinase signaling, The Journal of biological chemistry, vol.287, pp.7265-7278
Movement directionality in collective migration of germ layer progenitors, Current biology : CB, vol.20, pp.161-169, 2010. ,
E-cadherin regulates cell movements and tissue formation in early zebrafish embryos. Developmental dynamics : an official publication of the, American Association of Anatomists, vol.230, pp.263-277, 2004. ,
Heterooligomerization of reggie-1/flotillin-2 and reggie-2/flotillin-1 is required for their endocytosis, Cellular signalling, vol.21, pp.1287-1297, 2009. ,
Increased activity of mitogen activated protein kinase pathway in flotillin-2 knockout mouse model, Cellular signalling, vol.26, pp.198-207, 2014. ,
Forces driving epithelial spreading in zebrafish gastrulation, Science, vol.338, pp.257-260, 2012. ,
2-O-sulfotransferase regulates Wnt signaling, cell adhesion and cell cycle during zebrafish epiboly, Development, vol.139, pp.1296-1305, 2012. ,
The yolk syncytial layer in early zebrafish development, Trends in cell biology, vol.20, pp.586-592, 2010. ,
Shield formation at the onset of zebrafish gastrulation, Development, vol.132, pp.1187-1198, 2005. ,
Flotillin-1/reggie-2 traffics to surface raft domains via a novel golgi-independent pathway. Identification of a novel membrane targeting domain and a role for palmitoylation, The Journal of biological chemistry, vol.277, pp.48834-48841, 2002. ,
, , 2009.
, Reggies/flotillins regulate retinal axon regeneration in the zebrafish optic nerve and differentiation of hippocampal and N2a neurons, J Neurosci, vol.29, pp.6607-6615
Role of EGF-induced tyrosine phosphorylation of reggie-1/flotillin-2 in cell spreading and signaling to the actin cytoskeleton, Journal of cell science, vol.120, pp.395-406, 2007. ,
Zebrafish gastrulation: cell movements, signals, and mechanisms, International review of cytology, vol.261, pp.159-192, 2007. ,
alphaE-catenin regulates cell-cell adhesion and membrane blebbing during zebrafish epiboly, Development, vol.139, pp.537-546, 2012. ,
Reggie-1 and reggie-2, two cell surface proteins expressed by retinal ganglion cells during axon regeneration, Development, vol.124, pp.577-587, 1997. ,
Rapid reverse genetic screening using CRISPR in zebrafish, Nature methods, vol.12, pp.535-540, 2015. ,
, , 2005.
, E-cadherin is required for gastrulation cell movements in zebrafish, Mechanisms of development, vol.122, pp.747-763
The tight junction component Claudin E is required for zebrafish epiboly. Developmental dynamics : an official publication of the, American Association of Anatomists, vol.239, pp.715-722, 2010. ,
The epithelial cell adhesion molecule EpCAM is required for epithelial morphogenesis and integrity during zebrafish epiboly and skin development, PLoS genetics, vol.5, p.1000563, 2009. ,
Cleavage of E-cadherin by ADAM10 mediates epithelial cell sorting downstream of EphB signalling, Nat Cell Biol, vol.13, pp.1100-1107, 2011. ,
, , 2007.
, Reggie/flotillin proteins are organized into stable tetramers in membrane microdomains, Biochem J, vol.403, pp.313-322
Reggies/flotillins interact with Rab11a and SNX4 at the tubulovesicular recycling compartment and function in transferrin receptor and E-cadherin trafficking, Molecular biology of the cell, vol.24, pp.2689-2702, 2013. ,
Pou5f1-dependent EGF expression controls E-cadherin endocytosis, cell adhesion, and zebrafish epiboly movements, Developmental cell, vol.24, pp.486-501, 2013. ,
Glycosylphosphatidyl inositol-anchored proteins and fyn kinase assemble in noncaveolar plasma membrane microdomains defined by reggie-1 and -2, Molecular biology of the cell, vol.12, pp.3031-3045, 2001. ,
, Flotillin 2 knockdown is correlated with a reduction in Flotillin 1 expression
, B-Differential interference contrast images of zebrafish embryos at different stages (4-, 16-and 1000-cell stage, 75% epiboly, Prim-6 and 25
, C-The graph shows the percentage of embryos that completed a normal epiboly (white), of embryos that were strongly delayed and reached 60-75% epiboly (orange) and of embryos that were arrested/dead between 50-60% epiboly (red) at 9.5 hpf. The number of embryos analyzed for each condition (from at least three independent experiments) is
, Flotillin 1 (right panels) distribution in the dorsal part of zebrafish embryos at the indicated stages. Inserts show the entire embryos. Bar = B-The distance (B) between the DC margin and the F-actin, D-Representative confocal images of Flotillin
MoATGFlot2) embryos. Some cells are colored to follow their movement in the layer during the recording period. Stars indicate a DC inserted in the migrating layer ,
, D-Trajectories of DCs in control and Flotillin MOs during 20 min
, DCs in control and Flotillin MOs during 20 min
duration was measured in DCs of control (MoCtrl) and Flotillin MOs (MoATGFlot2) during ,
MoCtrl) and Flotillin MO (MoATGFlot2) embryos were co-injected with GFP-CAAX ,
, Shown are y-z projections of images from a 15-minute time-lapse recording to visualize cell tracking during the recording period. The lines indicate cell trajectories. The graph represents the trajectories of all cells
Boxplots represent the quantification of the total speed (H), instantaneous speed (I), net speed (J) and persistence (K) in control (MoCtrl) and Flotillin MOs (MoATGFlot2) embryos during 30 min ,
, L-Schematic representation of the different speeds measured in H, I and J
MoATGFlot2 (n=54 cells from n = 10 embryos) embryos from at least three independent experiments. From H to K: MoCtrl (n=41 cells Figure 3: Flotillins knock-down impairs E-cadherin and ?-catenin accumulation at cell-cell contacts of DCs A-Zebrafish embryo injected with a mRNA encoding GFP-tagged Flotillin 2 and stained using an anti-E-cadherin antibody and Hoescht (nuclei) ,
, Flotillin 2 and E-cadherin signals were highlighted using the «Colocalization Threshold» tool from Image J and are shown in white. Pearson's coefficient = 0.64. Bar = 10 µm
, DCs at 75% epiboly in control (MoCtrl) and Flotillin MO (MoATGFlot2) embryos by confocal microscopy. Bar = 10 µm
, Intensity profiles of E-cadherin and ?-catenin signals in control (MoCtrl)(black) and
MoATGFlot2)(red) embryos were measured along lines (as shown in figure B) by line scanning ,
Boxplots represent the normalized intensity values of the F-actin, E-cadherin and ?-catenin signals at cell-cell contacts (CCC) in control (MoCtrl) and Flotillins MO (MoATGFlot2) embryos ,
, H-Quantification of the F-actin, E-cadherin and ?-catenin signal at CCC and outside CCC (NCC)
, Shown is the CCC signal/NCC signal ratio in control (MoCtrl) and Flotillins MO (MoATGFlot2) embryos
For E-cadherin: n = 20 cells from n = 12 MoCtrl embryos ,
, For ?-catenin: n = 14 cells from n = 8 MoCtrl embryos
?-catenin and actin expression assessed by immunoblotting in protein extracts from control (MoCtrl) and Flotillins MO (MoATGFlot2) embryos (50 embryos were pooled for each condition) ,