E. Gomez-diaz and J. Figuerola, New perspectives in tracing vector-borne interaction networks, Trends Parasitol, vol.26, pp.470-476, 2010.

K. D. Mccoy, The population genetic structure of vectors and our understanding of disease epidemiology, Parasite, vol.15, pp.444-452, 2008.

F. Keesing, R. D. Holt, and R. S. Ostfeld, Effects of species diversity on disease risk, Ecol Lett, vol.9, pp.485-98, 2006.

A. M. Kilpatrick, P. Daszak, M. J. Jones, P. P. Marra, and L. D. Kramer, Host heterogeneity dominates West Nile virus transmission, Proc Biol Sci, vol.273, pp.2327-2360, 2006.

R. J. Kent, Molecular methods for arthropod bloodmeal identification and applications to ecological and vector-borne disease studies, Mol Ecol Resour, vol.9, pp.4-18, 2009.

W. R. Mukabana, W. Takken, and B. Knols, Analysis of arthropod bloodmeals using molecular genetic markers, Trends Parasitol, vol.18, pp.505-514, 2002.

M. Alcaide, C. Rico, S. Ruiz, R. Soriguer, J. Munoz et al., Disentangling vector-borne transmission networks: a universal DNA barcoding method to identify vertebrate hosts from arthropod bloodmeals, PLoS One, vol.4, 2009.

B. F. Allan, L. S. Goessling, G. A. Storch, and R. E. Thach, Blood meal analysis to identify reservoir hosts for Amblyomma americanum ticks, Emerg Infect Dis, vol.16, pp.433-473, 2010.

R. Buitrago, S. Depickere, M. F. Bosseno, E. S. Patzi, E. Waleckx et al., Combination of cytochrome b heteroduplex-assay and sequencing for identification of triatomine blood meals, Infect Genet Evol, vol.12, pp.21-28, 2012.
URL : https://hal.archives-ouvertes.fr/hal-01256057

C. B. Graham, W. C. Black, K. A. Boegler, J. A. Montenieri, J. L. Holmes et al., Combining real-time polymerase chain reaction using SYBR green I detection and sequencing to identify vertebrate bloodmeals in fleas, J Med Entomol, vol.49, pp.1442-52, 2012.

N. Haouas, B. Pesson, R. Boudabous, J. P. Dedet, H. Babba et al., Development of a molecular tool for the identification of leishmania reservoir hosts by blood meal analysis in the insect vectors, Am J Trop Med Hyg, vol.77, pp.1054-1063, 2007.

F. Kirstein and J. S. Gray, A molecular marker for the identification of the zoonotic reservoirs of Lyme borreliosis by analysis of the blood meal in its European vector Ixodes ricinus, Appl Environ Microbiol, vol.62, pp.4060-4065, 1996.

J. K. Meece, C. E. Reynolds, P. J. Stockwell, T. A. Jenson, J. E. Christensen et al., Identification of mosquito bloodmeal source by terminal restriction fragment length polymorphism profile analysis of the cytochrome B gene, J Med Entomol, vol.42, pp.657-67, 2005.

E. Pettersson, S. Bensch, M. Ander, J. Chirico, R. Sigvald et al., Molecular identification of bloodmeals and species composition in Culicoides biting midges, Med Vet Entomol, vol.27, pp.104-116, 2013.

L. Eisen, R. Lane, J. Gray, O. Kahl, R. S. Lane et al., Vectors of Borrelia burdorferi sensu lato, Lyme Borreliosis: biology, epidemiology and control, pp.91-115, 2002.

V. Cotté, S. Bonnet, M. Cote, and M. Vayssier-taussat, Prevalence of five pathogenic agents in questing Ixodes ricinus ticks from Western France, Vector Borne Zoonotic Dis, vol.10, pp.723-753, 2010.

G. Stanek and M. Reiter, The expanding Lyme Borrelia complex-clinical significance of genomic species?, Clin Microbiol Infect, vol.17, pp.487-93, 2011.

B. Pichon, D. Egan, M. Rogers, and J. Gray, Detection and identification of pathogens and host DNA in unfed host-seeking Ixodes ricinus L. (Acari: Ixodidae), J Med Entomol, vol.40, pp.723-754, 2003.

M. C. Scott, J. R. Harmon, J. I. Tsao, C. J. Jones, and G. J. Hickling, Reverse line blot probe design and polymerase chain reaction optimization for bloodmeal analysis of ticks from the eastern United States, J Med Entomol, vol.49, pp.697-709, 2012.

W. Radstrom and P. , Purification and characterization of PCR-inhibitory components in blood cells, J Clin Microbiol, vol.39, pp.485-93, 2001.

A. Al-soud, W. Jonsson, L. J. Radstrom, and P. , Identification and characterization of immunoglobulin G in blood as a major inhibitor of diagnostic PCR, J Clin Microbiol, vol.38, pp.345-50, 2000.

A. Akane, K. Matsubara, H. Nakamura, S. Takahashi, and K. Kimura, Identification of the heme compound copurified with deoxyribonucleic-acid (DNA) from bloodstains, a major inhibitor of polymerase chain-reaction (PCR) amplification, J Forensic Sci, vol.39, pp.362-72, 1994.

A. S. Raikhel, The intestine, Raikhel AS, Hoogstraal H, editors. An atlas of ixodid tick ultrastructure, pp.59-97, 1983.

B. I. Tarnowski and L. B. Coons, Ultrastructure of the midgut and blood meal digestion in the adult tick dermacentor variabilis, Exp Appl Acarol, vol.6, pp.263-89, 1989.

P. F. Humair, V. Douet, F. M. Cadenas, L. M. Schouls, I. Van-de-pol et al., Molecular identification of bloodmeal source in Ixodes ricinus ticks using 12S rDNA as a genetic marker, J Med Entomol, vol.44, pp.869-80, 2007.

F. Morán-cadenas, O. Rais, P. F. Humair, V. Douet, J. Moret et al., Identification of host bloodmeal source and Borrelia burgdorferi sensu lato in fieldcollected Ixodes ricinus ticks in Chaumont (Switzerland), J Med Entomol, vol.44, pp.1109-1126, 2007.

B. Pichon, M. Rogers, D. Egan, and J. Gray, Blood-meal analysis for the identification of reservoir hosts of tick-borne pathogens in Ireland, Vector Borne Zoonotic Dis, vol.5, pp.172-80, 2005.

S. Bonnet, M. Jouglin, L. Malandrin, C. Becker, A. Agoulon et al., Transstadial and transovarial persistence of Babesia divergens DNA in Ixodes ricinus ticks fed on infected blood in a new skin-feeding technique, Parasitology, vol.134, pp.197-207, 2007.

S. Bonnet and X. Y. Liu, Laboratory artificial infection of hard ticks: a tool for the analysis of tick-borne pathogen transmission, Acarologia, vol.52, pp.453-64, 2012.
URL : https://hal.archives-ouvertes.fr/hal-01301271

X. Y. Liu, M. Cote, R. Paul, and S. I. Bonnet, Impact of feeding system and infection status of the blood meal on Ixodes ricinus feeding, Ticks Tick Borne Dis, vol.5, pp.323-331, 2014.

T. Hall, BIOEDIT: a user-friendly biological sequence alignment, editor and analysis program for Windows 95/98/NT, Nucleic Acids Symp Ser, vol.41, pp.95-103, 1999.

A. Untergasser, I. Cutcutache, T. Koressaar, J. Ye, B. C. Faircloth et al., Primer3-new capabilities and interfaces, Nucleic Acids Res, vol.40, p.115, 2012.

R. C. Edgar, MUSCLE: multiple sequence alignment with high accuracy and high throughput, Nucleic Acids Res, vol.32, pp.1792-1799, 2004.

P. E. Nielsen, M. Egholm, R. H. Berg, and O. Buchardt, Sequence-selective recognition of DNA by strand displacement with a thymine-substituted polyamide, Science, vol.254, pp.1497-500, 1991.

K. N. Ganesh and P. E. Nielsen, Peptide nucleic acids: analogs and derivatives, Curr Org Chem, vol.4, pp.931-974, 2000.

L. Novere and N. , MELTING, computing the melting temperature of nucleic acid duplex, Bioinformatics, vol.17, pp.1226-1233, 2001.

J. Santalucia, H. T. Allawi, and A. Seneviratne, Improved nearest-neighbor parameters for predicting DNA duplex stability, Biochemistry (Mosc), vol.35, pp.3555-62, 1996.

O. M. Amin and D. E. Sonenshi, Development of American Dog tick, DermacentorVariabilis, following partial feeding by immatures, Ann Entomol Soc Am, vol.63, pp.128-161, 1970.

H. G. Koch, Development of the lone star tick, Amblyomma-Americanum (acari, ixodidae), from immatures of different engorgement weights, J Kans Entomol Soc, vol.59, pp.309-322, 1986.

D. E. Sonenshine, Biology of ticks, vol.2, 1993.

M. J. Crawley, The R book, 2007.

B. M. Bolker, Ecological models and data in R, 2008.

F. Kirstein and J. S. Gray, Blood meal identification in ticks: a promising tool in ecological research on tick-borne diseases, Zentralblatt Bakteriol-Int J Med Microbiol Virol Parasitol Infect Dis, vol.289, pp.760-764, 1999.

C. S. Apperson, H. K. Hassan, B. A. Harrison, H. M. Savage, S. E. Aspen et al., Host feeding patterns of established and potential mosquito vectors of West Nile virus in the eastern United States, Vector Borne Zoonotic Dis, vol.4, pp.71-82, 2004.

M. F. Bosseno, L. S. Garcia, F. Baunaure, E. M. Gastelum, M. S. Gutierrez et al., Short report: Identification in triatomine vectors of feeding sources and Trypanosoma cruzi variants by heteroduplex assay and a multiplex minjexon polymerase chain reaction, Am J Trop Med Hyg, vol.74, pp.303-308, 2006.

M. E. Woods, J. A. Montenieri, R. J. Eisen, N. S. Zeidner, J. N. Borchert et al., Identification of flea blood meals using multiplexed real-time polymerase chain reaction targeting mitochondrial gene fragments, Am J Trop Med Hyg, vol.80, pp.998-1003, 2009.

A. Estrada-pena, J. J. Osacar, B. Pichon, and J. S. Gray, Hosts and pathogen detection for immature stages of Ixodes ricinus (Acari : Ixodidae) in North-Central Spain, Exp Appl Acarol, vol.37, pp.257-68, 2005.

M. Dietrich, E. Lobato, T. Boulinier, and K. D. Mccoy, An experimental test of host specialisation in a ubiquitous polar ectoparasite: a role for adaptation?, J Anim Ecol, vol.83, pp.576-87, 2014.

R. Galun and S. Sternberg, Effects of host spectra on feeding-behavior and reproduction of soft ticks (Acari Argasidae), Bull Entomol Res, vol.68, pp.153-160, 1978.

K. D. Mccoy, E. Leger, and M. Dietrich, Host specialization in ticks and transmission of tick-borne diseases: a review, Front Cell Infect Microbiol, vol.3, 2013.

P. Willadsen, D. H. Kemp, and R. V. Mckenna, Bloodmeal ingestion and utilization as a component of host specificity in the tick, Boophilus-Microplus. Z Parasitenkd-Parasitol Res, vol.70, pp.415-435, 1984.

E. C. Loomis, Rearing of Boophilus-Microplus (Acarina-Ixodidae) on laboratory rabbit, Ann Entomol Soc Am, vol.64, p.598, 1971.

J. L. Brunner, L. Cheney, F. Keesing, M. Killilea, K. Logiudice et al., Molting success of Ixodes scapularis varies among individual blood meal hosts and species, J Med Entomol, vol.48, pp.860-866, 2011.

K. Kuramochi, Survival, ovarian development and bloodmeal size for the horn fly Haematobia irritans irritans reared in vitro, Med Vet Entomol, vol.14, pp.201-207, 2000.

J. M. Venzal and A. Estrada-pena, Larval feeding performance of two Neotropical Ornithodoros ticks (Acari: Argasidae) on reptiles, Exp Appl Acarol, vol.39, pp.315-335, 2006.

T. D. Gariepy, R. Lindsay, N. Ogden, and T. R. Gregory, Identifying the last supper: utility of the DNA barcode library for bloodmeal identification in ticks, Mol Ecol Resour, vol.12, pp.646-52, 2012.

M. A. Oshaghi, A. R. Chavshin, and H. Vatandoost, Analysis of mosquito bloodmeals using RFLP markers, Exp Parasitol, vol.114, pp.259-64, 2006.

P. Bize, C. Jeanneret, A. Klopfenstein, and A. Roulin, What makes a host profitable? Parasites balance host nutritive resources against immunity, Am Nat, vol.171, pp.107-125, 2008.

L. C. Harrington, J. D. Edman, and T. W. Scott, Why do female Aedes aegypti (Diptera: Culicidae) feed preferentially and frequently on human blood, J Med Entomol, vol.38, pp.411-433, 2001.

M. Sarfati, B. R. Krasnov, L. Ghazaryan, I. S. Khokhlova, L. J. Fielden et al., Energy costs of blood digestion in a host-specific haematophagous parasite, J Exp Biol, vol.208, pp.2489-96, 2005.

D. E. Sonenshine, The midgut, Biology of ticks, vol.1, pp.159-88, 1991.

W. R. Mukabana, W. Takken, P. Seda, G. F. Killeen, W. A. Hawley et al., Extent of digestion affects the success of amplifying human DNA from blood meals of Anopheles gambiae (Diptera: Culicidae), Bull Entomol Res, vol.92, pp.233-242, 2002.

M. A. Oshaghi, A. R. Chavshin, H. Vatandoost, F. Yaaghoobi, F. Mohtarami et al., Effects of post-ingestion and physical conditions on PCR amplification of host blood meal DNA in mosquitoes, Exp Parasitol, vol.112, pp.232-238, 2006.

S. Steuber, A. Abdel-rady, and P. H. Clausen, PCR-RFLP analysis: a promising technique for host species identification of blood meals from tsetse flies (Diptera: Glossinidae), Parasitol Res, vol.97, pp.247-54, 2005.

U. A. Laskay, L. Breci, I. Vilcins, G. Dietrich, A. G. Barbour et al., Survival of host blood proteins in Ixodes scapularis (Acari: Ixodidae) ticks: a time course study, J Med Entomol, vol.50, pp.1282-90, 2013.

O. Önder, W. G. Shao, B. D. Kemps, H. Lam, and D. Brisson, Identifying sources of tick blood meals using unidentified tandem mass spectral libraries, Nat Commun, vol.4, 2013.

O. Önder, W. G. Shao, H. Lam, and D. Brisson, Tracking the sources of blood meals of parasitic arthropods using shotgun proteomics and unidentified tandem mass spectral libraries, Nat Protoc, vol.9, pp.842-50, 2014.

O. Schmidt, H. Dautel, J. Newton, and J. S. Gray, Natural isotope signatures of host blood are replicated in moulted ticks, Ticks Tick Borne Dis, vol.2, pp.225-232, 2011.

S. Wickramasekara, J. Bunikis, V. Wysocki, and A. G. Barbour, Submit your next manuscript to BioMed Central and take full advantage of: ? Convenient online submission ? Thorough peer review ? No space constraints or color figure charges ? Immediate publication on acceptance ? Inclusion in PubMed, CAS, Scopus and Google Scholar ? Research which is freely available for redistribution, Emerg Infect Dis, vol.14, pp.1273-1278, 2008.