Transmission of Leishmania infantum in the Canine Leishmaniasis Focus of Mont-Rolland, Senegal: Ecological, Parasitological and Molecular Evidence for a Possible Role of Sergentomyia Sand Flies

Abstract : Leishmania (L.) infantum is the causative agent in an endemic focus of canine leishmania-sis in the Mont-Rolland district (Thiès, Senegal). In this area, the transmission cycle is well established and more than 30% of dogs and 20% of humans are seropositive for L. infan-tum. However, the sand fly species involved in L. infantum transmission cycle are still unknown. Between 2007 and 2010, 3654 sand flies were collected from different environments (indoor, peridomestic, farming and sylvatic areas) to identify the main L. infantum vector(s). Nine sand fly species were identified. The Phlebotomus genus (n = 54 specimens ; Phlebotomus (Ph) duboscqi and Phlebotomus (Ph). rodhaini) was markedly under-represented in comparison to the Sergentomyia genus (n = 3600 specimens; Sergentomyia (Se) adleri, Se. clydei, Se. antennata, Se. buxtoni, Se. dubia, Se. schwetzi and Se. magna). Se. dubia and Se. schwetzi were the dominant species indoor and in peridomestic environments, near humans and dogs. Blood-meal analysis indicated their anthropophilic behavior. Some Se. schwetzi specimens fed also on dogs. The dissection of females in the field allowed isolating L. infantum from sand flies of the Sergentomyia genus (0.4% of Se. dubia and 0.79% of Se. schwetzi females). It is worth noting that one Se. dubia female not engorged and not gravid revealed highly motile metacyclic of L. infantum in the anterior part of the midgut. PCR-based diagnosis and sequencing targeting Leishmania kinetoplast DNA (kDNA) highlighted a high rate of L. infantum-positive females (5.38% of Se. dubia, PLOS Neglected Tropical Diseases |
Liste complète des métadonnées

Littérature citée [42 références]  Voir  Masquer  Télécharger

https://hal.umontpellier.fr/hal-02003302
Contributeur : Philip Agnew <>
Soumis le : vendredi 1 février 2019 - 11:29:43
Dernière modification le : mercredi 29 mai 2019 - 10:56:23
Document(s) archivé(s) le : jeudi 2 mai 2019 - 15:51:20

Fichier

journal.pntd.0004940.PDF
Fichiers éditeurs autorisés sur une archive ouverte

Identifiants

Citation

Massila Senghor, Abdoul Niang, Jérôme Depaquit, Hubert Ferté, Malick Faye, et al.. Transmission of Leishmania infantum in the Canine Leishmaniasis Focus of Mont-Rolland, Senegal: Ecological, Parasitological and Molecular Evidence for a Possible Role of Sergentomyia Sand Flies. PLoS Neglected Tropical Diseases, Public Library of Science, 2016, 10 (11), pp.e0004940. ⟨10.1371/journal.pntd.0004940⟩. ⟨hal-02003302⟩

Partager

Métriques

Consultations de la notice

48

Téléchargements de fichiers

67