I. Antibodies-for, All primary antibodies used are listed as follows: Rabbit anti-Crb3 (D2, kind gift from Dr A.Le Bivic, IBDML, France, 1:200) Mouse anti-Moesin (610401, BD transduction, 1:100) Mouse anti-AnkG (NB20, Calbiochem, 1:50) Rabbit anti-Gliomedin (KR34, Koch, 1:1,000) Mouse anti-KV1, Antibodies incorporated 1:500) Rabbit anti-pERM, vol.2, p.100

, :1,000) Rabbit anti-Nav1.8 (AP672-4.1, kind gift of SR Levinson, university of Colorado Health Center, USA, 1:200) Rabbit anti-Caspr/paranodin (L51, kind gift from Dr L. Goutebroze, Institut du Fer a ` Moulin, Institut du Fer a ` Moulin, vol.1, p.0, 0200.

, Molecular Probes) was used. To visualize mouse primary antibodies: Alexa Fluor F(ab')2 fragments of goat anti mouse IgG (H þ L) 488, To visualize rabbit primary antibodies: Alexa Fluor donkey anti rabbit IgGs (H þ L) 488, 594 or 647 (A21206, A21207, A31573 respectively, vol.594

, Rabbit anti-ERLI (UM510) and Rabbit anti-CLPI (UM377, 1:250-500) were a kind gift from Dr Margolis

, 1:1,000) Rabbit anti-phospho-YAP (S127A) (#4911, Cell Signaling, 1:1,000) Rabbit anti-GFP (A11122, Invitrogen, 1:500) Mouse anti-FLAG (F-3165, Sigma, 1:500) Rabbit anti-MAG (#9086, Cell Signaling, 1:1,000) Goat anti-MPZ (PA5-18773, Thermo Fisher, 1:3,000) Rabbit anti-Rab11a (#2413, Cell Signaling, 1:1,000) Rabbit anti-lamining1 (NBP1-19643, Novus Biologicals

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