Structural organization of the perimysium in bovine skeletal muscle: Junctional plates and associated intracellular subdomains

Abstract : We analyzed the structural features of the perimysium collagen network in bovine Flexor carpi radialis muscle using various sample preparation methods and microscopy techniques. We Wrst observed by scanning electron microscopy that perimysium formed a regular network of collagen Wbers with three hierarchical levels including (i) a loose lattice of large interwoven Wbers ramiWed in (ii) numerous col-lagen plexi attaching together adjacent myoWbers at the level of (iii) speciWc structures that we call perimysial junctional plates. Second, we looked more closely at the intracellular organization underneath each plate using transmission electron microscopy, immunohisto-chemistry, and a three-dimensional reconstruction from serial sections. We observed the accumulation of myonuclei arranged in clusters surrounded by a high density of subsarcolemmal mitochondria and the proximity of capillary branches. Third, we analyzed the distribution of these perimysial junctional plates, subsarcolemmal mitochondria, and myonuclei clusters along the myoWbers using a statistical analysis of the distances between these structures. This revealed a global colocalization and the existence of adhesion domains between endomysium and perimysium. Taken together, our observations give a better description of the perimysium organization in skeletal muscle, and provide evidence that perimysial junctional plates with associated intracellular subdomains may participate in the lateral transmission of contractile forces as well as mechanosensing.
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E. Passerieux, R. Rossignol, A. Chopard, A. Carnino, J.F. Marini, et al.. Structural organization of the perimysium in bovine skeletal muscle: Junctional plates and associated intracellular subdomains. Journal of Structural Biology, Elsevier, 2006, 154 (2), pp.206 - 216. ⟨10.1016/j.jsb.2006.01.002⟩. ⟨hal-01758589⟩

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