Real-time impedance-activated dielectrophoretic actuation for reconfigurable manipulation of single flowing particles
Résumé
This work presents an innovative all-electrical platform for
selective single-particle manipulation. The platform combines
microfluidic impedance cytometry for label-free particle
characterization and dielectrophoresis for contactless multi-way
particle separation. The microfluidic chip has a straightforward
coplanar electrode layout and no particle pre-focusing mechanism is
required. An original online algorithm analyzes the impedance
signals of each incoming particle and regulates in real time the
dielectrophoretic voltages according to a desired control logic. As
a proof-of-concept, three operation modes are demonstrated on a
mixture of 8, 10, and 12 μm diameter beads: (i) particle position
swapping across the channel axis, irrespective of particle size,
(ii) size-based particle separation, irrespective of the particle
position, and (iii) sorting of a selected sequence of particles. As
a perspective, the versatility of impedance cytometry and
dielectrophoresis and the possibility of configurin alternative
control logics hold promise for Advanced particle and cell
manipulation
Origine | Fichiers produits par l'(les) auteur(s) |
---|