Skip to Main content Skip to Navigation
Book sections

In vivo imaging of microtubule organization in dividing giant cell

Abstract : Mitosis which is a major step during plant development can also be observed in physiopathological conditions. During the compatible interaction between the root-knot nematode Meloidogyne incognita and its host Arabidopsis, the pathogen induce through repeated divisions without complete cytokinesis the formation of hypertrophied and multinucleate feeding cells, named giant cells. Due to the presence of hypertrophied plant cell material surrounding the giant cells, classical live cell imaging gave therefore very poor resolution. Here, we describe a protocol which allows the in vivo observation of the mitotic apparatus in developing giant cells using confocal imaging of vibrosliced tissues. This approach can also be used to visualize in vivo other cellular processes occurring in different steps of giant cells.
Document type :
Book sections
Complete list of metadata

https://hal.inrae.fr/hal-02801847
Contributor : Migration Prodinra Connect in order to contact the contributor
Submitted on : Thursday, October 14, 2021 - 12:13:10 PM
Last modification on : Wednesday, November 3, 2021 - 6:55:31 AM

File

Caillaud_MMB 2016.pdf
Files produced by the author(s)

Identifiers

Collections

Citation

Marie-Cécile Caillaud, Bruno Favery. In vivo imaging of microtubule organization in dividing giant cell. Plant cell division: methods and protocols, 1370, Humana Press Inc., 2016, Methods in Molecular Biology, 978-1-4939-3142-2; 978-1-4939-3141-5. ⟨10.1007/978-1-4939-3142-2_11⟩. ⟨hal-02801847⟩

Share

Metrics

Record views

47

Files downloads

26