Skip to Main content Skip to Navigation
Journal articles

Five-Years Review of RHCE Alleles Detected after Weak and/or Discrepant C Results in Southern France

Abstract : Immunohematology laboratories are regularly facing transfusion issues due to serological weaknesses. Altered (partial) RH antigens account for most of them. In some situations, RHCE variant alleles are involved. Herein we present our three-step molecular exploration, with allele frequencies, that has efficiently untangled RH2 phenotype weaknesses and discrepancies in our 2017–2021 cohort. In the last 5 years, the PACA Corse EFS molecular platform received 265 samples from healthy blood donors or patients with C and C/e typing difficulties. The first-intention technique (DNA array and real time PCR for RHCE*CeRN research) detected RHCE variant alleles in 143 cases (54%). The RHCE alleles classically found in African populations were the most frequent, with RHCE*CeRN allele in 40 cases (15%) and (C)ces haplotype type 1 and 2 in 26 cases (10%). A “CE” effect haplotype was suspected in 56 cases, due to the uncommon DCE haplotype that may explain the low C expression. When there were no RHCE*Ce or RHCE*CE alleles, we then searched for RHD polymorphisms by DNA array. We detected the RHD*DAU5 and RHD*DIVa in 18 and 7 cases respectively, suggesting that C ambiguity is related to the presence of these alleles which has never been described with DAU5. If no variant RHCE and RHD alleles were detected, we finally sequenced the 10 exons of both RHCE and RHD genes according to the clinical context and found seven new RHCE alleles. Thus, this molecular strategy would improve the knowledge of RHCE variants’ expression and, thus, optimize the transfusion management.
Complete list of metadata

https://hal.archives-ouvertes.fr/hal-03695279
Contributor : Stéphane Mazières Connect in order to contact the contributor
Submitted on : Tuesday, June 14, 2022 - 4:16:25 PM
Last modification on : Wednesday, June 15, 2022 - 3:36:56 AM

File

Pedini_Genes22.pdf
Publisher files allowed on an open archive

Identifiers

Collections

Citation

Pascal Pedini, Lugdivine Filosa, Nelly Bichel, Christophe Picard, Monique Silvy, et al.. Five-Years Review of RHCE Alleles Detected after Weak and/or Discrepant C Results in Southern France. Genes, MDPI, 2022, 13 (6), pp.1058. ⟨10.3390/genes13061058⟩. ⟨hal-03695279⟩

Share

Metrics

Record views

48

Files downloads

2